1 March 02, 2014 1
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Aquipuribacter nitratireducens sp. nov., a 3
novel bacterium isolated from a soil sample 4
of mud volcano, Andaman Islands, India.
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Srinivas, T. N. R.
1, Anil Kumar, P.
1, Tank, M.
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Sunil, B.
1, Poorna Manasa
1, Zareena Begum
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Shivaji S.
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1Centre for Cellular and Molecular Biology,
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Uppal Road, Hyderabad-500 007, INDIA
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2Leibniz-Institut für Meereswissenschaften, IFM-
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GEOMAR, Marine Mikrobiologie, Düsternbrooker
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Weg 20, D-24105 Kiel, Germany
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Running title Aquipuribacter nitratireducens sp. nov.
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Address for correspondence *Dr S Shivaji
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Centre for Cellular and Molecular Biology
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Uppal Road, Hyderabad-500 007, INDIA
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Email: shivas@ccmb.res.in
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Telephone: 00-91-40-27192504
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Fax: 00-91-40-27160311
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Subject category New taxa (Actinobacteria)
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of
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strain AMV4T is FN397670.
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Supplementary Fig. S1. Two-dimension thin-layer chromatogram of the total lipids of
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Aquipuribacter nitratireducens AMV4T. The TLC plate was sprayed with
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molybdatophosphoric acid.The first dimension was run from left to right and the second
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dimension from bottom to the top. Abbreviations: PG, phosphatidylglycerol; GL,
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unidentified glycolipid; PL, unidentified phospholipid; L, unidentified lipid. The the spots
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were identified as phospho-, amino- or glyco-lipids by spraying with molybdenum blue,
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ninhydrin and –napthol reagents respectively.
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Supplementary Fig. S2. Neighbour-joining phylogenetic tree, based on 16S rRNA gene
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sequences, showing the distant relationships between strain AMV4T and representatives
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of the species belongs to the family Intrasporangiaceae, Numbers at the nodes are
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bootstrap values >50%. Micrococcus luteus DSM 20030T was used as an out group.
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Bar, 0.01 substitutions per nucleotide position.
