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Characterization of the Fatty Acid Composition of

Nannochloropsis salina as a Determinant of Biodiesel Properties

Nagwa Gamal-ElDin Mohammady

Department of Botany and Microbiology, Faculty of Science, Moharam Bey 21511, Alexandria University, Alexandria, Egypt. Fax: +203 3911 794.

E-mail: nagwa_phyco@yahoo.com

Z. Naturforsch. 66 c, 328 – 332 (2011); received November 21, 2010/March 27, 2011

Nannochloropsis salina was cultured batch-wise to evaluate the potential of the alga to produce biodiesel. The cells were harvested at the end of the exponential growth phase when the concentration was 18 · 106 cells/mL culture. The growth estimated as dry weight from this cell number was (3.8 r 0.7) mg/L. The lipid and triglyceride contents were 40%

and 12% on a dry weight basis, respectively. The amount of the ratio triglycerides/total lipids was approximately 0.3.

The composition of triglyceride fatty acid methyl esters (biodiesel) was analysed by gas- liquid chromatography and identifi ed as: C14:0, C16:0, C16:1, C18:0, C18:1, C18:2, C18:3, C20:1, and C20:5. The ratio of unsaturated to saturated fatty acid contents was approximate- ly 4.4. Additionally, the characterization of each individual fatty acid ester was discussed with regard to the fuel properties of biodiesel produced by the alga.

Key words: Biodiesel, Lipids, Nannochloropsis salina

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