Isolation of Quality Total RNA from the Aromatic Plant Origanum onites

There was nearly complete recovery of the total protein, total free amino acid, lactate, nu- cleic acid (DNA and RNA), and pyruvate level, the activity of cytochrome oxidase,

Good-quality total RNA from leaves of Avicen- nia germinans could be easily isolated within 2 h by this protocol which avoided the limitation of plant materials and could provide

been some reports on total RNA isolation from mangroves (Fu et al., 2004) an optimized protocol to provide high quantities of pure total RNA for gene expression studies was lacking..

First, the exact string matching using suffix trees or arrays does not exploit the properties of the well-formed RNA structure strings; a bottom-up processing of the tree also

During outgrowth from stationary phase, 6S RNA itself serves as a template for transcription of small "product" RNAs (pRNAs) [8, 9] which results in the dissociation of the

*) unknown.. Table 2 SAMPL and AFLP primer combinations used for genotyping42 accessions of O.. Trait abbreviations: DF drug fraction, EOY essential oil yield, CAC carvacrol content

A) Western blot analysis of S100 and P100 fractions of the overexpressing strain. Equal volume amounts of the S100 and the P100 fractions were separated in 12% SDS-PAGE, blotted

30 L of sterile RNase-free water was pipetted directly on the membrane and allowed to incubate for 1 minute, after which the RNeasy spin column was centrifuged at 10,000 rpm or