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Figure S1: Caliper picture RNA quality check
Figure S2: Gel picture of amplified cDNA a) prior to and b) after normalization a)
Lane 1 Lane 2 Lane 3
b)
Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6
Lane 1: DNA Ladder mix Gene Ruler Fermentas 0.5 ug/ul Lane 2: empty
Lane 3: no DSN
Lane 4: DSN treatment (undiluted Enzyme) Lane 5: 1:2 diluted DSN treatment
Lane 6: 1:4 diluted DSN treatment
Figure S3: Size-selection
mRNA above 600 bp was cut from gel, followed by RNA-seq protocol.
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