label GMYC OTU country / locality GPS species GenBank EL001 eastern ujhelyii PL / brook, Góry Świętokrzyskie 50°52′34″N 20°58′41″E E. cf. ujhelyii MH093830 EL002 eastern ujhelyii PL / brook, Zimny Dól reservation 50°4′6″N 19°40′45″E E. cf. ujhelyii MH093831 EL007 eastern ujhelyii SK / Bukovinský brook, Šiatorská Bukovinka 48°11′3″N 19°50′35″E E. cf. ujhelyii MH093832 EL008 lateralis SK / Brusnička brook, Brusnica 49°9′21″N 21°42′35″E E. lateralis MH093833 EL009 lateralis SK / Rieka brook, Lendak 49°14′44″N 20°20′37″E E. lateralis MH093834 EL011 western ujhelyii HU / brook, Aszófö 46°56′2″N 17°49′51″E E. cf. ujhelyii MH093835 EL012 western ujhelyii CZ / Babský brook, Řevnice 49°54′7″N 14°13′38″E E. cf. ujhelyii MH093836 EL013 eastern ujhelyii CZ / brook, Ponova Louka 50°52′27″N 14°14′29″E E. cf. ujhelyii MH093837 EL014 western ujhelyii CZ / brook, Nišovice 49°9′9″N 13°53′16″E E. cf. ujhelyii MH093838 EL015 lateralis CZ / Dvorský brook, Labský důl 50°45′5″N 15°34′52″E E. lateralis MH093839 EL016 lateralis CZ / Bílé Labe river, Dívčí Lávky 50°44′32″N 15°36′32″E E. lateralis MH093840 EL017 affinis CZ / Losínský brook, Kácov 49°47′18″N 15°1′3″E E. affinis MH093841 EL018 affinis CZ / Střela river, Borek 49°54′56″N 13°30′51″E E. affinis MH093842 EL019 lateralis CZ / Bílý brook, Smědava 50°50′35″N 15°16′34″E E. lateralis MH093844 EL020 western ujhelyii CZ / Výřice brook, Nuzice 49°15′43″N 14°27′4″E E. cf. ujhelyii MH093845 EL021 western ujhelyii CZ / Výřice brook, Nuzice 49°15′43″N 14°27′4″E E. cf. ujhelyii MH093846 EL022 eastern ujhelyii CZ / Klíčava river, Lány 50°6′15″N 13°53′0″E E. cf. ujhelyii MH093847 EL024 western ujhelyii CZ / Šembera river, Doubravčice 50°1′12″N 14°48′53″E E. cf. ujhelyii MH093848 EL025 western ujhelyii CZ / Klíčava river, Lány 50°6′15″N 13°53′0″E E. cf. ujhelyii MH093849 EL026 eastern ujhelyii CZ / Bratřejovka brook, Bratřejov 49°13′34″N 17°55′1″E E. cf. ujhelyii MH093850 EL027 eastern ujhelyii CZ / Bratřejovka brook, Bratřejov 49°13′34″N 17°55′1″E E. cf. ujhelyii MH093851 EL028 western ujhelyii CZ / Jevišovka springs, Komárovice 49°3′36″N 15°41′27″E E. cf. ujhelyii MH093852 EL029 western ujhelyii CZ / Zlatý brook, Záhoří 48°56′55″N 14°5′30″E E. cf. ujhelyii MH093853 EL030 affinis CZ / Střela river, Ondřejov 49°58′0″N 13°20′44″E E. affinis MH093843 EL031 western ujhelyii CZ / Krasetínský brook, Krasetín 48°52′56″N 14°18′54″E E. cf. ujhelyii MH093854 EL032 eastern ujhelyii CZ / Radějovka brook, Radějov 48°51′11″N 17°21′56″E E. cf. ujhelyii MH093855 EL033 lateralis CZ / Čistá brook, Černý důl 50°38′31″N 15°42′47″E E. lateralis MH093856 EL034 western ujhelyii CZ / Jihlava river, Horní Ves 49°17′31″N 15°18′25″E E. cf. ujhelyii MH093857 EL040 eastern ujhelyii PL / brook, Świnna Poręba 49°49′50″N 19°31′12″E E. cf. ujhelyii MH093858 EL041 eastern ujhelyii SK / Čamovský brook, Čamovce 48°14′56″N 19°53′38″E E. cf. ujhelyii MH093859 EL042 eastern ujhelyii SK / Krtíš river, Riečky 48°17′31″N 19°17′30″E E. cf. ujhelyii MH093860 EL043 eastern ujhelyii SK / Čerešňový brook, Kľačany 48°28′0″N 18°16′42″E E. cf. ujhelyii MH093861 EL044 eastern ujhelyii SK / Šútovský brook, Kocúrany 48°46′50″N 18°32′14″E E. cf. ujhelyii MH093862 EL045 eastern ujhelyii SK / Žilina reservoir tributary, Strčno 49°11′9″N 18°50′35″E E. cf. ujhelyii MH093863 EL046 eastern ujhelyii SK / Prosiek brook, Prosiek 49°11′1″N 19°29′36″E E. cf. ujhelyii MH093864 EL048 eastern ujhelyii SK / Sikenica river, Malé Krškany 48°13′19″N 18°40′23″E E. cf. ujhelyii MH093865 EL049 eastern ujhelyii SK / Vydrica river, Bratislava 48°10′27″N 17°4′29″E E. cf. ujhelyii MH093866 EL050 lateralis SK / Chvojnica brook, Lopašov 48°45′5″N 17°20′0″E E. lateralis MH093867 EL051 eastern ujhelyii SK / Drietomica river, Žítková 48°58′12″N 17°53′60″E E. cf. ujhelyii MH093868 EL052 eastern ujhelyii SK / Drietomica river, Drietoma 48°57′27″N 17°54′38″E E. cf. ujhelyii MH093869 EL063 quadrilineata CZ / Radějovka brook, Strážnice 48°52′51″N 17°18′55″E E. quadrilineata MH093874 EL065 lateralis CZ / Úpa river, Pec pod Sněžkou 50°42′48″N 15°43′26″E E. lateralis MH093870 EL066 lateralis CZ / Úpa river, Pec pod Sněžkou 50°42′48″N 15°43′26″E E. lateralis MH093871 EL067 lateralis CZ / Bílé Labe river, Jelení Boudy 50°44′52″N 15°36′53″E E. lateralis MH093872 EL068 quadrilineata CZ / Sudoměřický brook, Mlýnky 48°50′38″N 17°18′48″E E. quadrilineata MH093875
EL069 quadrilineata CZ / Sudoměřický brook, Mlýnky 48°50′38″N 17°18′48″E E. quadrilineata MH093876 EL070 eastern ujhelyii CZ / Klimentský brook, Vřesovice 49°4′46″N 17°12′48″E E. cf. ujhelyii MH093877 EL071 eastern ujhelyii CZ / Kyjovka river, Staré Hutě 49°7′35″N 17°16′15″E E. cf. ujhelyii MH093878 EL072 western ujhelyii CZ / Šembera river, Doubravčice 50°1′12″N 14°48′52″E E. cf. ujhelyii MH093879 EL073 eastern ujhelyii CZ / Křepička brook, Dolní Dunajovice 48°58′12″N 16°7′3″E E. cf. ujhelyii MH093880 EL074 eastern ujhelyii CZ / Vítovický brook, Vítovice 49°13′14″N 16°50′38″E E. cf. ujhelyii MH093881 EL075 western ujhelyii CZ / Klaperův brook, Čížov 48°52′20″N 15°52′54″E E. cf. ujhelyii MH093882 EL076 western ujhelyii CZ / Dobročovický brook, Třebohostice 50°1′49″N 14°42′57″E E. cf. ujhelyii MH093883 EL077 western ujhelyii CZ / Lipižský brook, Dobříš 49°47′57″N 14°9′42″E E. cf. ujhelyii MH093884 EL078 western ujhelyii CZ / Voznický brook, Voznice 49°49′23″N 14°12′32″E E. cf. ujhelyii MH093885 EL079 eastern ujhelyii CZ / Křtinský brook, Křtiny 49°17′44″N 16°44′20″E E. cf. ujhelyii MH093886 EL080 eastern ujhelyii CZ / Mojena brook, Přílepy 49°19′5″N 17°38′19″E E. cf. ujhelyii MH093887 EL081 eastern ujhelyii CZ / Rakovec brook, Bukovinka 49°18′1″N 16°50′17″E E. cf. ujhelyii MH093888 EL082 eastern ujhelyii CZ / Kazivec brook, Suchovské Mlýny 48°53′16″N 17°34′37″E E. cf. ujhelyii MH093889 EL083 eastern ujhelyii CZ / Zhrta brook, Jankovice 49°21′20″N 17°37′44″E E. cf. ujhelyii MH093890 EL084 eastern ujhelyii CZ / Hrubý brook, Javorník 48°50′43″N 17°33′24″E E. cf. ujhelyii MH093891 EL085 eastern ujhelyii CZ / Járkovec, Radějov 48°51′44″N 17°23′50″E E. cf. ujhelyii MH093892 EL087 western ujhelyii CH / Moille Saugeon brook, Moille Saugeon 46°34′45″N 6°42′5″E E. cf. ujhelyii MH093893 EL088 eastern ujhelyii CZ / Loučka brook, Loučka u Litovle 49°39′50″N 17°0′24″E E. cf. ujhelyii MH093894 EL089 eastern ujhelyii CZ / Veverka brook, Veveří 49°15′15″N 16°27′42″E E. cf. ujhelyii MH093895 EL090 western ujhelyii CZ / brook, Bransouze 49°18′30″N 15°45′36″E E. cf. ujhelyii MH093896 EL091 eastern ujhelyii CZ / brook, Číchov 49°16′42″N 15°45′8″E E. cf. ujhelyii MH093897 EL092 quadrilineata AT / Schaubach brook, Purgstall 48°4′8″N 15°6′42″E E. quadrilineata MH093873 EL093 western ujhelyii AT / Schaubach brook, Purgstall 48°4′8″N 15°6′42″E E. cf. ujhelyii MH093898
Table S1. A list of all analysed specimens with country and locality specification and GenBank
accession numbers for cox1 mitochondrial gene sequences. Used abbreviations: CZ – Czech Republic,
SK – Slovakia, HU – Hungary, AT – Austria, PL – Poland, CH – Switzerland.
E. a ffinis E. a n ta lyen sis E. ca lab ra E. fa llax E. g a lil ea e E. g ra n d iae E. g ride lli i E. h ybla ea E. lat er a lis E. lun a ris E. m a lic kyi E. q u a d rili n ea ta E. u jh elyi i
N_PLP
min 19 0 6 1 7 2 5 10 7 12 3 25 13
mean 34.72 2.03 9.97 4.56 11.09 5.70 16.23 15.83 12.94 18.60 7.14 36.00 20.18
max 54 6 14 8.50 19 14.50 34 27 23 28 10.50 45 27
N_PLH
min 0 0 0 0 0 0 0 0 0 0 0 NA 0
mean 4.57 0 0 0 0 0 0 0 0 0 0 NA 0
max 10 0 0 0 0 0 0 0 0 0 0 NA 0
N_OUT
min 0 6.50 3 3 0 0 2 0 0 0 0 13 18
mean 0.02 12.96 8.21 8.44 0 0.47 12.02 0.08 2.75 1.60 0 14.30 33.50
max 1 24 16.50 22 0 2.50 22.50 1 10 6 0 16 46
N_CBS
min 13.50 13 17 18 19 16 14 15 15 14 18 15 17
mean 17.31 15.93 19.12 21.76 19.77 18.35 18.91 17.59 17.48 17.60 20.80 15.80 20.42
max 20 18 23 26 21.50 21 25 20 21 20 24.50 16 23.50
N_TCB
min 10 8 10 10 13 6.50 7 8 7 5 12 7 9.50
mean 12.03 10.34 12.19 13.15 14.55 8.58 10.18 11.29 8.44 6 13.98 7.50 13.32
max 15 12 14.50 17.50 16 11.50 13 14 13 7 18 8 16
N_CLW
min 2 4 2 2 1 2 2 2 1 1 2 2 2
mean 3.02 6.66 2.02 2.03 2.36 2 2.04 2 1 1 2 2 2.58
max 4 9 3 3 4 2 3 2 1 1 2 2 4
N_BVF
min 1 1 1 >6 6 NA NA 1 1 1 NA 30 19
mean 1.13 1 1 NA 22.45 1 1 1 1 1 1 34 29
max 3 1 3 NA 36 2 NA 1 NA 3 NA 38 45
N_HFF
min 8 0 0 0 3 0 0 0 0 0 0 0 0
mean 27.18 0 0 0 3 0 0 0 0 0 0 0 0
max 58.00 0 NA NA 3 NA NA NA NA NA NA 0 NA
R_1GI
min 1.37 1.32 2.28 2.23 1.80 2.01 1.88 2.25 1.80 1.76 2.12 2.10 1.53 mean 1.80 1.60 2.81 2.86 2.12 2.78 2.32 2.73 2.15 2.24 2.58 2.30 1.90 max 2.34 1.81 3.87 3.43 2.41 3.87 2.87 3.28 2.67 2.93 3.26 2.70 2.67 R_7GI
min 1.52 1.74 2.16 2.69 2.30 2.00 1.58 2.09 1.65 1.73 1.87 2.40 1.80 mean 1.94 2.03 2.78 3.51 2.61 2.62 2.31 2.66 2.09 2.07 2.42 2.40 2.30 max 2.62 2.35 3.69 4.38 2.81 3.16 3.22 3.21 2.75 2.54 2.89 2.40 2.89 R_LBR
min 4.07 4.18 3.88 4.07 3.84 3.04 4.14 4.15 4.87 4.79 3.92 4.10 4.66 mean 4.69 4.79 4.54 4.56 4.16 4.87 4.83 4.55 5.46 5.63 4.33 4.50 4.88
max 5.52 5.96 4.89 6 4.51 6.05 5.41 4.88 7.40 6.74 4.79 5 5.33
R_GLA
min 2.59 2.91 2.88 2.57 2.96 2.39 2.93 2.75 2.70 2.15 2.78 2.90 2.70 mean 2.96 3.66 3.12 2.91 3.21 2.75 3.37 3.23 3.18 3.10 3.07 3.00 2.96 max 3.39 4.51 3.54 3.19 3.78 3.17 4.16 3.80 4.63 4.00 3.54 3.10 3.46 R_GLB
min 2.45 2.25 2.23 2.49 2.54 2.65 2.35 2.51 2.22 2.25 2.44 2.70 2.56 mean 2.81 2.60 2.64 2.78 2.81 2.96 2.68 2.72 2.71 2.62 2.68 2.80 2.84 max 3.18 2.94 2.97 3.12 3.06 3.35 3.07 3.10 3.09 3.19 2.95 3.00 3.01
S_HLB for details see legend 1 1_2 1 1 1_2 1 1 1 0 0 1 1 0
S_TAR for details see legend C A A NA A NA NA A NA A NA A C
S_HEM for details see legend C A NA NA C NA NA NA NA NA NA NA NA
S_BFE for details see legend P P P NA S S NA S P P NA S S
Table S2. Numerical and qualitative characters of European Electrogena species based on available
publications. Minimum, mean and maximum values for each numerical character are listed. Four
qualitative characters are listed: S_HLB (1 means hairs overlapping over the tip of hypopharynx, 0
means hairs not overlapping over the tip of hypopharynx and 1_2 means intermediate state between
1 and 0 – hairs overlapping over the tip of hypopharynx are present, but short), S_TAR (A means
distally darkened tarsus, B means proximally darkened tarsus and C means whole dark tarsus), S_HEA
(for abbreviations see 2.5.1.) and S_BFE (P means pointed bristles, S means spatulate or rounded
bristles). For abbreviations of numerical and qualitative characters see Appendix.
AFFINIS LATERALIS QUADRILINEATA EASTERN UJHELYII WESTERN UJHELYII min mean max min mean max min mean max min mean max min mean max N_LBB 12 15.15 20 12 17.07 22 14 15.40 18 12 16.12 20 14 16.80 24 N_CBS 11 12.92 14 12 15.33 17 11 12.00 13 14 15.09 17 13 14.93 18 N_TCB 10 10.60 11 9 10.75 12 10 10.00 10 10 12.26 15 10 11.55 15
N_OUT 0 0.27 2 1 3.33 6 6 10.40 17 5 12.03 22 5 13.57 21
N_PLP 12 30.55 48 5 10.07 16 13 17.00 21 10 16.17 26 7 14.55 23 N_PLS 40 54.60 79 24 33.47 45 29 34.60 45 27 36.94 48 18 34.67 48
N_PLH 0 2.60 6 0 0 0 0 0.00 0 0 0.24 2 0 0.23 1
N_PLBas 6 13.60 20 4 6.47 10 5 8.40 10 3 6.90 11 2 7.10 13
N_VEN 1 4.20 8 3 6.80 11 13 17.40 27 0 3.42 10 0 3.86 8
N_MPB_L 4 5.69 7 5 6.21 8 6 6.80 7 4 6.52 8 5 6.81 10
N_MPB_R 3 4.92 6 4 5.23 6 5 5.80 7 4 5.38 7 4 6.03 8
N_LPH_prox 26 36.83 49 8 12.50 21 16 18.40 21 10 14.36 20 9 14.02 20
N_LPH_dist 2 5.46 8 1 4.27 7 7 9.20 11 3 5.45 9 2 4.44 6
R_LBR 4.48 5.10 5.73 4.87 5.42 6.39 4.85 5.09 5.39 3.69 5.17 5.87 4.53 5.08 5.86 R_GLA 2.59 3.09 3.79 2.72 3.11 3.92 3.07 3.35 3.75 2.94 3.49 5.81 2.84 3.25 4.27 R_GLB 2.71 2.98 3.25 2.69 2.96 3.15 2.74 2.85 2.96 2.48 2.83 3.03 1.94 2.87 3.08
N_CLW 3 3.07 4 1 1.08 2 2 2 2 2 2.76 3 2 3.11 6
N_BVF 0 0.14 1 0 0.79 1 29 35.6 40 12 26.33 35 13 25.95 43
N_HFF 15 23.21 52 0 0.07 1 0 0 0 0 0 0 0 0.12 2
R_3FEM 2.64 3.00 3.46 2.74 2.96 3.36 2.58 2.86 3.07 2.23 2.70 3.20 2.24 2.66 3.14 R_1GI 1.61 1.80 2.08 2.06 2.29 2.66 2.10 2.36 2.60 1.90 2.17 2.50 1.70 2.14 2.57 R_4GI 1.05 1.14 1.24 1.09 1.22 1.42 1.15 1.18 1.20 1.13 1.23 1.33 1.12 1.23 1.33 R_7GI 1.57 1.84 2.17 1.88 2.30 2.63 2.46 2.65 2.86 2.22 2.56 3.04 1.94 2.41 3.10
