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Kinetics of Total Enzymatic Hydrolysis of Acetylcholine and Acetylthiocholine Pavla Zdrazˇilova´

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Kinetics of Total Enzymatic Hydrolysis of Acetylcholine and Acetylthiocholine

Pavla Zdrazˇilova´

a

, Sˇa´rka Sˇteˇpa´nkova´

a

, Martina Vra´nova´

a

, Karel Komers

a,

*, Alena Komersova´

a

, and Alexander C ˇ egan

b

a Faculty of Chemical Technology, Department of Physical Chemistry, University of Pardubice, na´m. Cˇ s. legiı´ 565, 532 10 Pardubice, Czech Republic. Fax: +42 04 66 03 70 68.

E-mail: karel.komers@upce.cz

b Department of Biological and Biochemical Sciences, University of Pardubice, Sˇtrossova 239, 530 02 Pardubice, Czech Republic

* Author for correspondence and reprint requests

Z. Naturforsch.61 c, 289Ð294 (2006); received August 29/October 10, 2005

Kinetics and the mechanism of totalin vitrohydrolyses (i.e.up to the exhaustion of sub- strate) of acetylcholine and acetylthiocholine by acetylcholinesterase and butyrylcholinester- ase were studied in vitro in a batch reactor at 25∞C, pH 8 and ionicstrength of 0.11m.

Every hydrolysis was monitored by 2Ð3 independent analytical methods. All studied types of enzymatichydrolyses fulfilled the MichaelisÐMenten reaction scheme with the irreversible second step. A table of obtained average values of rate constants and estimations of initial molar enzyme concentrations, and discussion of the results are presented.

Key words:Acetylcholine, Hydrolysis, Kinetics

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