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Determination of Selenium in Teucrium Spe- cies by Hydride Generation Atomic Absorp- tion Spectrometry

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Determination of Selenium in Teucrium Spe- cies by Hydride Generation Atomic Absorp- tion Spectrometry

Renata Jurisˇic´

a,*

, Sanda Vladimir-Knezˇevic´

a

, Zdenka Kalo era

a

, and Jerica Grgic´

b

a Facultyof Pharmacyand Biochemistry, Universityof Zagreb, A. Kovacˇic´a 1,

HR-10000 Zagreb, Croatia. Fax: +3 85-1-4 92 00 89.

E-mail address: renata@pharma.hr

b Institute of Public Health, F. Krezˇme 3, HR-31000 Osijek, Croatia

* Author for correspondence and reprint requests Z. Naturforsch.58 c, 143Ð145 (2003); received August 1/

September 23, 2002

Hydride generation atomic absorption spectrometry (HGAAS) was applied for determination of selenium content in dried aerial parts of wild and cultivated Teucriumspecies (Lamiaceae) growing in Croatia:T. ar- duini L.,T. chamaedrys L.,T. flavum L.,T. montanum L., T. poliumL., and T. scordiumL. subsp.scordioides Schreb. Special attention was paid to the wet oxidation procedure for the sample dissolution. The proposed pro- cedure involved microwave-assisted sample digestion using a mixture of HNO3/H2O2. Wild specimens gen- erallyhad a higher content of selenium, with concentra- tions of 0.030Ð0.095 mg/kg of the drydrug. Cultivated plants contained 0.020Ð0.055 mg Se/kg.

Key words: Teucrium, Selenium, Hydride Generation Atomic Absorption Spectrometry

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