1st day
0 20 40 60 80 100 120
8:0010:0012:0014:00 16:0018:00
Effective quantum yield (% of PAR)
PA PAB
15th day
08:0010:0012:0014:00 16:0018:00 Time of day (h)
28th day
08:0010:0012:00 14:0016:0018:00
UV on UV on UV on
a
a a a
b
b b
b b
b a,b
a,b
1500 2000 2500 3000 3500 4000
0 5000 10000 15000 20000
Time (min) Surface area (pixel) 0
1 2 3 4 5 6
PARPAB
Growth rate (% day
-1)
PAB PAR a
b 0.00
0.05 0.10 0.15 0.20 0.25 0.30 0.35 0.40 0.45
Chl a Fucox
mg g FW-1
PAR a PAB
0.00 0.01 0.02 0.03 0.04 0.05 0.06 0.07 0.08 0.09 0.10
Chl c1 ß-Carot
b a
b a
b a
b
Pump (Eheim, Type 1060, 38L/m) 80L Tank Basin= 600x400x120mm PVC pipes= 120mmx70mm
Filter UVC-lamp
SW inflow 3 White Flourescent lamp (Osram) 3 UV lamps (Q-Panel UVA-340)
GG 400WG320Quartz
Water inflow Water outflow
LABORATORY INCUBATION SYSTEM 1 LABORATORY INCUBATION SYSTEM 2 Growth
Chamber with Automated Online Video Measuring Technique
PHOTOSYNTHESIS
GROWTH GERMINATION
DNA DAMAGE PIGMENT
SCIENTIFIC QUESTION:
How does UV radiation affects growth, as an integrative parameter of all physiological processes, in determining distribution pattern of macrolagae and its impact on recruitment in nearshore ecosystems ?
Fig. 4. Germination experiment on
Laminaria hyperboreazoospores Fig. 5. Germination experiment on
Mastocarpus stellatusspores
PAR
PAR+UVA
PAR+UVA+UVB
PAR
PAR+UVA
PAR+UVA+UVB
a
b c
d
e f g
h i
j k l
Fig. 7. ISITECTMgrowth chamber showing: (a) camera control panel; (b) CCD camera; (c) timer; (d) sliding platform; (e) lamps; (f) infrared diodes; (g) UV transparent Plexiglas chamber; (h) cut-off filter glasses;
(i) PC; (j) water reservoir; (k) water inflow and (l) water outflow.
Fig. 10. Effects of UVR on Chondrus crispus include lower growth rate and thallus bleaching.
RECRUITMENT ?
Fig. 8. Morphological responses of Laminaria ochroleucaafter 28 days UVR exposure include: (a) tissue deformation; (b) lesion; (c) blistering; (d) bleaching and (e) thickening of the meristematic lamina.
Fig. 9. Growth rate of L. ochroleucameasured in the growth chamber using a video image analysis software (MedeaLABTM).
Fig. 6. Flow-through basin incubation system installed inside a temperature controlled room.
a a
b
c d
e
e Fig. 1. Photosynthesis of youngLaminaria ochroleucasporophytes showed partial acclimation to chronic UVR exposure
Fig. 2. Chronic UVB exposure significantly reduced all pigments in youngL. ochroleucasporophytes
Fig. 3. Example of DNA blots to determine DNA damage using immunoassay for CPDs, showing accumulation of number of CPDs in UV exposed samples (dark dots).
UV (-)
PAR (+) Effects of UV-radiation on photosynthesis, growth and recruitment in marine macroalgae
Michael Y. Roleda
1,3, Dieter Hanelt
1and Christian Wiencke
21
Biologische Anstalt Helgoland, Alfred Wegener Institute for Polar and Marine Research, D-27498 Helgoland, Germany
2
Projectgroup: Solar UV-radiation, Foundation Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany
3
Biology Department, De La Salle University, 2401 Taft Avenue, 1004 Manila, Philippines
PAR= Photosynthetically active radiation PA = PAR+UVA PAB=
PAR+UVA+UVB
