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Effect of differently conserved herbage on chemical composition of forages and nitrogen turnover in dairy cows

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Agroscope | 2018

Effect of differently conserved herbage on chemical composition of forages and nitrogen turnover in dairy cows

U. Wyss1, C. Böttger2, F. Dohme-Meier1, K.-H. Südekum2

1Agroscope, Ruminant Research Unit, 1725 Posieux, Switzerland

2University of Bonn, Institute of Animal Science, Bonn, Germany

Method of conservation affected crude protein composition of the forage. Feeding only forage derived from herbage in late lactation resulted in high urinary N losses and an N deficiency in dairy cows. The absolute excretion of urinary N was highest when cows were fed silage, which had more non-protein-N than the two other forages.

Material and Methods

Feeding maximum forage to ruminants involves less competition with human food resources. Forages derived from herbage are often high in rumen-degradable crude protein (CP), resulting in poor nitrogen (N) utilisation by ruminal microbes and N losses to the environment. However, the mode of conservation can influence the CP fractions in forage and, thereby, the potential N utilisation by the animal. The objective of the present study was to determine the effect of three types of forage preservation on CP fractions and N efficiency in dairy cows.

Ley, mainly composed of:

Lolium perenne, Trifolium repens andT. pratense

34-d regrowth Cut 30 August 2016

Forage conservation

Analysis of non-protein N, buffer soluble N and fibre-bound N and calculation of CP fractions A, B1, B2, B3, C (assumed to decrease in ruminal solubility in this order)

Silage (SI) 24 wilting

Baling at 56% DM Ventilated hay (VH) 27 h wilting

68% DM

Ventilation to 88% DM Field-dried hay (FH) 72 h drying on the field 86% DM

Replicated 3 × 3 Latin Square 6 multiparous Holstein cows (milk yield 23.5 ± 3.9 kg/d; 270.3 ± 7.2 d in milk)

Adaptation period (14 d) Collection period (7 d)

• Feed intake 0.95 of ad libitum intake in adaptation

• Milk yield and composition

• Total collection of urine and faeces

• Ruminal fluid and blood collection on d 2 and 7 before the morning feeding

N balance trial

* Effect of conservation method Background

Results

Conclusions

SI VH FH P-value*

Feed intake (kg DM/d) 17.3a 19.2b 17.9ab 0.05

Faecal N (% of total N intake) 30a 31a 35b <0.01

Urinary N (% of total N intake) 55 52 49 0.55

Ruminal ammonia (mmol/L) 7.4ab 8.2a 7.0b 0.04

Plasma urea (mmol/L) 7.2a 7.2a 6.4b <0.01

Milk urea (mg/kg) 370a 351a 306b <0.01

Table 1. Daily intake of dry matter (DM) and relative excretion of N in urine and faeces as well as concentrations of ruminal ammonia and plasma and milk urea

Fig. 1. Crude protein fractions in the fresh herbage and the conserved forage. CP, crude protein, A, B1, B2, B3, C, CP fractions according to Sniffen et al. (1992)

Figure 2. Daily N intake and N excretion via milk, faeces and urine and N balance.

Milk yield (19.7 kg/d) as well as fat (4.93%) and protein (3.79%) percentage did not differ between treatments.

Analysis of variance:

conservation method as main factor

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