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Changes in Catecholamine Levels in Short Day-Induced Cotyledons of Pharbitis nil

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Changes in Catecholamine Levels in Short Day-Induced Cotyledons of Pharbitis nil

Masayuki Suzuki

a

, Masaya Mizoguchi

b

, Fumihiko Yano

b

, Utako Hara

b

, Mineyuki Yokoyama

c

, and Naoharu Watanabe

b*

a United Graduate School of Agricultural Science, Gifu University (Shizuoka University), 836 Ohya, Shizuoka 422Ð8529, Japan

b Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Ohya, Shizuoka 422Ð8529, Japan. Fax: +81-54-2 38-48 70.

E-mail: acnwata@agr.shizuoka.ac.jp

c Basic Resarch Center, Shiseido Co. Ltd., Yokohama 224-8558, Japan

* Author for correspnodence and reprint requests

Z. Naturforsch.58 c, 220Ð224 (2003); received October 29/November 25, 2002

We investigated the effects of catecholamine on flower-induction inP. nil(cv. Violet). GC- SIM analysis identified dopamine for the first time inP. nilseedlings. Dopamine levels in the cotyledons did not show a significant change during the inducing dark treatment. The dopamine content of cotyledons exposed to various durations of darkness were 0.1Ð0.2 nmol/

g fresh weight. The same content was found when cotyledons were exposed to continuous light.

Key words: Pharbitis nil, Dark Treatment, Catecholamine

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