• Keine Ergebnisse gefunden

Yeast Cell-Based Transport Assay for the Functional Characterization of Human 4F2hc-LAT1 and -LAT2, and LAT1 and LAT2 Substrates and Inhibitors.

N/A
N/A
Protected

Academic year: 2022

Aktie "Yeast Cell-Based Transport Assay for the Functional Characterization of Human 4F2hc-LAT1 and -LAT2, and LAT1 and LAT2 Substrates and Inhibitors."

Copied!
11
0
0

Wird geladen.... (Jetzt Volltext ansehen)

Volltext

(1)

source: https://doi.org/10.48350/158328 | downloaded: 31.1.2022

01234516 6 782319234332 4 14 265224 1 24

2729729 29 34 24 1329

! 4 3

"#$%&'(#)$%*+,%#),-%.%$/%0&10$%#,%&2

345676869:;<7:=>9?756@AB4CD:E9=8EB@D9C7=749FG47H9@576A:;<9@4F<9@4FIJ76K9@EB4C

LMNONNOPQOMRSPPTUVWSXYVZ[SONQM\ORQ]V^OMT[\^VS^T_X`aTbX`ac_dXef`gbOM]X`ah _dXef`ibj\^N WSVS^\]QNS^QRONQM\ORQ]V^OMT[\^VS^T_k`aTblQVWVWSOMRQPPO^Z[^\VSQM mnhWROM]O^SQMo\PoS]QMVWSRSPPpPO^p[VOqS\jT[SRQrRONQM\ORQ]TsaWSk`amnhWRYX`ac QTj\pM]p[^StpPOVS]QMoO^Q\pTROMRS^RSPPVZ[STUlWQPSmnhWRYX`ahQTOV^OMT[\^VS^j\^

M\MYROMRS^RSPPTsu^SRPQMQROPTVp]QSTWOoSWQtWPQtWVS]VWOVmnhWRYX`ac[POZTOMQN[\^VOMV

^\PSQMVpN\^[^\t^STTQ\M^S[^STSMVQMtOoOPQ]OMVQROMRS^VO^tSVse\MTSvpSMVPZURp^^SMV

^STSO^RWQTj\RpTQMt\MVWS]SoSP\[NSMV\j[\VSMVOM]T[SRQrRWpNOMmnhWRYX`ac QMWQwQV\^TsxMVWS\VWS^WOM]UmnhWRYX`ahQTSNS^tQMtOTVO^tSV\j\VWS^]QTSOTSTUVWpT OPT\tOQMQMtRPQMQROPQMVS^STVsa\]SVS^NQMSOjrMQVZOM]T[SRQrRQVZ\jTpwTV^OVSTOM]

QMWQwQV\^T j\^ mnhWRYX`ac \^ mnhWRYX`ahU ^\wpTV V^OMT[\^V RSPP OTTOZT O^S QM]QT[SMTOwPSsySWOoS\[VQNQzS]OM]oOPQ]OVS]OV^OMT[\^VOTTOZpTQMtRSPPT\jVWS NSVWZP\V^\[WQRZSOTV{7=>7B|B56:@75TVOwPZ\oS^S}[^STTQMtVWSWpNOMk`aTmnhWRYX`ac

\^YX`ahUOM]VWSX`aTX`ac\^X`ahOP\MSsaWS^O]Q\PQtOM]~k€XYPSpRQMSlOTpTS]OT

^S[\^VS^OM]VWSTpwTV^OVSTXYPSpRQMSUV^QQ\]\VWZ^\MQMS_abOM]VWZ^\}QMS_ambOTlSPPOTVWS QMWQwQV\^Tek OM]‚ukhƒ_„…aYƒgbj\^OTTOZoOPQ]OVQ\MsxwVOQMS]WOPjYNO}QNOP QMWQwQV\^ZR\MRSMV^OVQ\MTOPT\[^\oQ]S]MSl QMTQtWVTUSstsUQMV\VWSX`aT[SRQrRQVZ\j VWS[\VSMVQMWQwQV\^‚ukhƒOM]\MVWS[\VSMRZ\jVWSVWZ^\Q]W\^N\MSTaOM]amV\ QMWQwQVV^OMT[\^VVW^\ptWWpNOMmnhWRYX`ahsaWSX`acOM]X`ahOTTOZTO^S\j[O^VQRpPO^

QMVS^STVV\]SVS^NQMS[\TTQwPSQN[PQROVQ\MTOM]QM†pSMRST\jmnhWRQMPQtOM]wQM]QMtOM]

V^OMT[\^VsLMTpNNO^ZUVWS[^STSMVS]OTTOZTO^SoOPpOwPSj\^RWO^ORVS^QzOVQ\M\jPQtOM]TU SstsUV\lO^]TmnhWRYX`acT[SRQrRQVZUOM]ROMOPT\wSO[[PQS]j\^R\N[\pM]TR^SSMQMts nQMOPPZU\p^STVOwPQTWS]O[[^\ORWOM]OTTOZl\pP]OPT\wSO[[PQROwPSV\\VWS^k`aTOM]

X`aT\jQMVS^STVs

‡1ˆ93‰229423414Š‹ŒŽ‘’“’”•–—”5˜234332

™š•›šœž

Ÿ95B@934C7H9@7F G47H9@576A:;ŸBEB @7BF36BEA

¡›¢›£›šœž

DB489E{BEB=74F 345676869;:@¤959B@=>74<7:?9C7=749F I|B74

¥4C@9B5¦§¨4©9EF G47H9@576A:;<B59EFIJ76K9@EB4C

ª«–——›”“–¬š›¬›ž

­7?76@7:5®:67BC75 C7?76@7:5§;:67BC75¯7 ??§847 9§=>

°“›’±•”›•–¬ž

²>75B@67=E9JB558 ?7669C6:

Ÿ9EE8EB@<7:=>9?756@AF B59=67:4:;6>9³:8@4BE

®@:4679@574D:E9=8EB@<7:5=794=95

¡››¢›šž´µDB@=>¶´¶·

¸›“•›šž´µDBA¶´¶·

Ž¹œ±”‘›šž¶ºDBA¶´¶·

«•’•–¬ž

»B467|8C7IB4C®:67BC75­¼¶´¶·½

¾9B56Ÿ9EE¿<B59C²@B45|:@6¥55BA;:@

6>9®84=67:4BEŸ>B@B=69@7KB67:4:;

¦8?B4À®¶>=¿Á¥²·B4C¿Á¥²¶FB4C Á¥²·B4CÁ¥²¶I8 56@B695 B4C34>7 76:@

®@:46§D:E§<7:5=7§ºÂµÃµºÄÀ§

C:7·´§ÅźÆÇ;?:E §¶´¶·§µÃµºÄÀ

n^\MVQS^TQMÈ\PSRpPO^Q\TRQSMRSTÉlllsj^\MVQS^TQMs\^t ÈOZhƒhcÉÊ\PpNSiÉ`^VQRPSËfËigm ÌÍ!Î!ÏÍÐÐÍ5 [pwPQTWS]ÑhiÈOZhƒhc ]\QÑcƒsiÒÓjN\PwshƒhcsËfËigm

(2)

0 123456720 41

89 99 9 99999 9

!" #$$%&'( )*8 #$$+, '-9 99 !./",99 9!091%#2, ./"3994 59!"8 , /*9!/8 ,!61%%+&

71%#8,"8 99:;<=>?=@9:;<=>@

/8 9:;<=A?=>>B9CD<E>FB9:;<=>GB9CD<E>A

!71%#8,H"8 /8 74I999 999 /8 HH 99 J*9 ./"8 9+71!./"881&

"K$2,L8 !./"88#,!)01%%+, !, /8 !, 49 99

!M8 ,!"1%%#&N1%%#&) 01%%+&61%%+&71%#8, M8 !O1%%1&O 1%#+&J1%#P,

/8 #!./"38P,/8 1!./"382,99 /9Q+71!+71, -999!0

#$$2&)#$$$&.#$$$,79 +7194 4/8 #/8 1!0 1%1%,H/8 4 +719 9 *8!7RI#$$3& 9H1%%%&7 1%%P&'01%%2,+71*/8 #- !, * 9!71%#8&.1%#2&MST

"1%#$, -9I+71*

/8 # !MST"1%#$&)1%1%, H+71*/8 #9 9 UO

!JI1%%$, M8 I9 H+71*/8 1Q- 9-R 9VM8 9!LW1%%2&71%#8, 4/8 # I49/8 1 9*9

/8 # /8 19*

- 99 ** 9!61%%+&71%#8, 4M8 9+71*

/8 199 !)#$$$&O#$$$&'1%%1,U +71*/8 # */8 1 9 9 8 +!79 1%%#&X 1%#P, 9 1*

9*!11#,**1*-!L"M,!09 1%%2,49/

+71*/8 #*/8 1!0#$$2&.#$$$,U *Y)M1%8!0Z *%8P8,9 94+71*/8 # !U1%#%&Z1%#+&MST1%#2, 4+71*

/8 #*/8 19 H9 /8 19 UO"#

!091%##, /8 1*49 9 9 /8 1*

+71*

/8 #*/8 1 R -/8 [9999 - +71*/8 # */8 1* 9-M8 8 Q-/8 4 9 9

+71-/8

90\]EC^ 9M[01$8-- /8 #/8 1+719 M8 !01%#1,M[01$8

I9

9 /8 #!0 1%#1,99.1 9!'91%%2, 9_`Da`EbEc]de`c* 9 - 99 99!L1%#P,"

9 /8 !'1%#+&KI1%#3&"

1%1%,Z_^bEc]de`c!J

#$32, -99 9 R 9R _^bEc]de`c-9+71*

/8 #*/8 1f8Mg/*H M[01$8

!01%#1,+71*-/8 # /8 1 _`Da`E* 9+71 -M8 !"

1%#8&O1%#+&01%1%,H

hijklmniomkpjqnrsqtiumjormnkrnovwwwxyijklmniomkxjiz ‡ pt{|}|~vjqs€nv‚ilmrqnƒ„ƒ…†

ˆtklm‰sŠmtkŠhjlmtŠmo ‹‚ŒtkŠ‚ŒŒitko‰jil‚oot{o

(3)

012310456176897786655381350580468 665591738106354135 0120816

!"85060#$ %&'85660#$ &(0132360#$$)#

3508*56+003185534678134381589660+7031 78130124312310120158313433818 5667625450650123134385#

,-./01 -23-45,/.6753

89 :;< ;=>;?/@ABCDD< :;< ;EFGHIJKLM I:N 6OP>;QRSTUV2-.WX2-.WXQRSTUV2-.S>;?

2-.S

"8131896Y01Z501253186[\"]^+678

!6Y8356_73613`7a00Ybc1362_065)6678 01598Y03818978Y661defghif5031(bj Z 765

!6Y8356_73613`7a00Ybc1362_065)012 5667381 8978165*338631 6655381 6+65*05

698Y6205265734623126036+385k566987 012 !(0132360#$$)012987 012!"85060#$ %)#"68*0126655381 78123381589 781658+6665531 Y01 7 78012101598Y62d efghif(bj Z 765!7818)*05781276207782318 (01323lgmd!(0132360#$$)#'6531765*66 655612623101584996! n$Yb7831678326 Yb b" Yb "0" $Yb 35Zo[o_Z j#)78103131 n$p!+q+)7680126rst$$*0502u5628$#"65*66 58620v wx"139656#

y

z

6{ 2V2COU< ;C0>?< :9 < =>;?.B>;DA:B|-DD>}

8015852365 Y890*62defghif765!rst$$$) 665531678658123101586*66236231n$Y 0158499601266624761390381!%$$$~ nY31 88Y6Y606)#666*0561*0562465561231331 n$Y015849960124660316*053187626

R1€0/W‚ƒ„…†‡ˆ†‰†Šˆ†Š‹Œ‰‹Ž†‘’“”•–—˜™š‡›†Œœ„Š†„Š‹žŸ ¡¢£¤¥¡¦•§“˜œ†››¨†©‰ª†¨¨„Š«¬­®¯œ‡š°ƒ“–±³ªŠ«†™´š°ƒ“–±µ²³«ª††Š™´¬­®¯œ‡š°ƒ®

–±³¶›Œ†™´ªš°ƒ®–±³·„›†‹™’–—˜™š‡›†Œœ„Š†Œ‰‹Ž†„Š‹ŒŠ‹ªŠ¨ª…†ˆžŸ ¡¢£¤¥¡¦•§“˜œ†››¨„¨¨¯¸Š„Š¶›œŽ’°¨‹†ª„¨Ž¨„Šˆ„œ‹†‹¯†“‘…„Š‹„…†‰„Š‹¨›œ‹†ˆ„Š‹¯†

›„Š†ªª†«„…†¨œ¯¨†Šª‹¯†¨Œ¶¨†¹Œ†Š‹›º‰ª†¨†Š‹†ˆ‹ªŠ¨‰ª‹„Š¯„¶„‹„Š†©‰†ª„…†Š‹¨»R<=OBCDS¼½¾’¿‹‰„Š‹¨ª†ª†‰ª†¨†Š‹†ˆ¨…†ŠÀ¨‹Šˆªˆˆ†·„‹„Šª… ª†‰ª†¨†Š‹‹„·††©‰†ª„…†Š‹„Š‹ª„‰›„œ‹†’ÁŠ‹·„¨„¶›†´†ªªª¶ª¨ª†¨…››†ª‹¯Š¨º…¶›¨’

­ªŠ‹„†ª¨„Š•›†œŒ›ªÂ„¨œ„†Šœ†¨Ã¸¸¸’ªŠ‹„†ª¨„Š’ª« z •º®‘®“Ã…†Å𪋄œ›†Æ§ÆÅǬ

¦Š‹„‰Œˆ„Šˆ­‹„ˆ„¨ ˜°ƒŠˆš°ƒƒªŠ¨‰ª‹°¨¨º¨

(4)

0123456789463352823634462634212224 5282326374535487725363184275634 832596446305846453375 !"4463923

#64$253!22482363 2364%842 64 453548772543&'(7)*+,0-1./0!)/0!)-1.

/0!5/0!3834537521234567895:+) 120 453542;2562342522575263521463<827)=/

5462.223234> ?/.28163284@22;2562340ABCDEFG42 5214636;4852134632-=/12823633(=/84542 4256;>)(+=/.2816326@264> ?/.2816320025613 H622261#4/86&I3642#442?64216J1 146<6476K528463963J3/.28163213123454637 )=,242563463722142 1837542 4535425-1./0!)/0!)-1./0!5/0! 252 25752 8639 -=/ 1282363,=/ 7124645 846346772523413123454633)=/84542425 6; >)= /.281632 6@2 64 > ?/.281632 0025613 H622261#4/86&I3642#442?64 216J1146<64 76K5284639 63 J3/.281632 13123454637)=5/.2816323" 0.63611. 0).2432..15;6116,2;25623442124645 8463252413123454637)L)=/.2816320-1.

/0!)/0!)-1./0!3/0!3,L)= "

0-1./0!)3/0!)3)L,= " 0-1./0!3

/0!M22155236399563 ABCDEFNOP75216J1 1312345463 82 5 242563639 42 , <82 7 56118381QR >0#..63..0-.00,.

63..2323$ >? ;$.+..24;.,.

61523.5361 16? @33 :S!., 022T;52384Q831463UQI3642#4423 42456532!045664536323!-045;632 0#69#4/86&I3642#442-=/71282363 631842 64 ,=/ 7 124645 8463 4 67725234 131234546375(63 4, 8325964463 0,5

!256;25114T2357 859V253!2 84@26364642639)=/845424256;>)=

/.281632 6@2 64 > ?/.281632 0025613 H622 261#4/86&I3642#442?64216J1146<647 6K44252631842W=/7521463<82528463963 J3/.28163213123454637)=636931312345463 63)=/252)L,=0QR !3!-!2124645 252525263)X0<K<'#&!2J3'#&1312345463 ,X 0<K<63223234761J3845425 63664513123454638263423452 13463242213123454637'#&63&'(<82 6384@22;256234252+,75120-1./0!)/0!) -1./0!5/0!2;52639123834537521234567895 :+) 120453545214632523263521463482

YZ[\]^_`abcdefgfhijklgjmkmnopqfrsjkfnmhtrilkuvwtspoxyz{|}~mhlk€f‚oxyz{|ƒ~€hffk‚uvwtspoxyw{|„~…qrflkeoxyw{|†~‡jmqfgrˆjk€‰Š‹ŒŽ‘

’“”z•sfqqˆf–—hfˆˆjk€gtfsmhhfˆ—mkejk€ghlkˆ—mhgfh‡lhjlkgˆ˜™fgfhijkfeabcd‡lqrfˆlkešc›smkœefksfjkgfh‡lqˆbaˆžlhfjkejslgfe˜“flkŸˆglkelheef‡jlgjmkmn kmhilqj feelglnhmi gthffjkef—fkefkgf–—fhjifkgˆ‚flstlgqflˆgjkghj—qjslgf‚lhfˆtm¡k˜ankmg‡jˆj…qf‚fhhmh…lhˆlhfˆilqqfhgtlkˆ¢i…mqˆ˜

vhmkgjfhˆjk“mqfsrqlh£jmˆsjfksfˆ¤¡¡¡˜nhmkgjfhˆjmh€ ¨ “l¢wdwz¤¥mqrif¦¤xhgjsqf§”§¦cu

’lkgj—rejlkevmgjlejˆ •xylkeoxyyhlkˆ—mhgxˆˆl¢ˆ

(5)

012234567897937445376409729 37637629122345678979374 2!3"

#37337435837402!33867$$3$$"554

%&'68273($$3574"26788373$$"#373725$"32735 876 3883774"837743"622357364"64)%(#3$$ %% $""*37*$372$306744+$71,967449-.9 435/3"450 *$7641#3$$#"#"35#

$683(6$574"26788376736038733756$4532$3

#"34573560374129453!"53#""3$35#

3!/3$0437!41$3795$9679 435/3"372235 756$45#"73$3"35556468&'"4$$646!$ 0676/489437!41$3795$9679 435/3"63

#3$$94532$362"53#""3$35#62"3$6(7 4$"

0437!41$379 5$9 679 435 /3"9 86$$6#35 464 86714 45 972 037637 62912234567897937464"#3733"735 43#3$$8674#"4$$64643706264-,9 437!41$3795$9679 435/3"

9:; :<=:> ?@A @BCDEFGHA ; ; A =I:=JK; :; A @; A L@

123734"#37323786735$3"472$3+6754$""9 3"4$683474"86735MNOPQRSTUQ3$$"#""735876 374"26737"4$66434374"267"4$V876 733453234534323734"#373!34W4 3 683"3 323734"934374"267"4$"#37303735453$8

$4676434764 0W0$3"6866$66"

0'($343 45 3376$66" 039X4191945 8 '($34374"26762364323734"#3735337435

*4"65$653$70363"351037323734$ 5264#"344505$$467$Y35"436773"26454 22372$3 0$3"0393*35 22372$3 0$3 6773"2645"6Z/4$39467$Y355264"876 3 733453234534323734"#3730373545"65$

653$703#"*356354675376643W

0$3"47"%07245/68#73#""3586754$""

[\K]^_K

48+('79'798+('767'7#3733273""354 33$67623"MNOPQRSTUQ53"6#35427306"73267"

4664$37"8+('745('79$"63$

"4"'745'74"343684$$72763473276237$ 86$53596773$78*!35632$"3743458464$4 MNOPQRSTUQ0`6"3$$3$98ab4253$9

74"26703"#37353374353"7432!368 3""73c1d'($34346MUefUP3$$"gV%23273""4 36773"26454767'73(67"3323734""6#35

$377(45'7(5323453474"26703"9##373 3743c1d'($3432!346474"86735

Hhi][\jklmnopqrqstuvwruxvxyzm{yxs|}twv~€|‚ƒ„…†‡ˆŠxswv‹qŒƒ„…†‡ˆŽ‰Š‹sqqvŒ~€|‚ƒ„…€‡ˆŠ}qŒwvpƒ„…€‡ˆŠ‘uqrŒ}’uv‹“”•–—˜™š›—

œž†{q’qŸ sq’’uv‹r|qxssq’ xvpuv‹rswv’ xsrqs‘wsuwvr’¡¢qrqstuvqplmno‘w}q’wvp£n¤xv¥pqvquvrqs‘w’¦ml’§wsquvpuwrqp¡qwv¨’rwvpwsppq‘uwruxvxy vxstwu©qppwrwysxt r|sqquvpq qvpqvrqŸ qsutqvr’qw|wrqw’ruvrsu uwrqwsq’|xªv¡lyvxr‘u’uqqssxsws’wsq’twqsr|wv’«tx’¡

sxvruqs’uvxq}wszux’uqvq’¬ªªª¡ysxvruqs’uxs‹ ° w«€o€†¬­x}tq®¬„sruq¯ž¯®n~

œwvru }puwvpxruwpu’ {„…wvpƒ„……swv’ xsr„’’w«’

(6)

01234566278966425223311305321314522 212555545216321378955 315535 522165562141521541!3"#$22 523!351!%!&'65225126(535!5)534 !53527*3536+,+,-3)522(3!521.,!

75305655!52/3!5132435(2352)2 8941521012314456623'5,,,67553)5 13052258536(525355 5!532309:+04;"#$."#$.

9:+04;"#$+"#$+789<=/>

?5535!530506;! !6031(414533127@7,2 1";6545(01!1611241!5331166132135 32'35@7,65255++AB 79:+04;"#$.CAB 7"#$.+.,AB 79:+04;"#$+DCAB 7"#$+789=

#25126(51357*3536+,+,41!211 @7,2435!1631(5543130505(239:+1 6032320402!12323)53559:+04;"#$+

"#$+789=#356311305(523;4566253213 22(3030522335";6545789<=525 631230552452(235!"3213031E@F 7*!536+,,G3059:+04;"#$.254H4031IJF+,D 7'536+,.,?5135@7,65216+AB 79:+04;

"#$.6GAB 7"#$..C7AB 79:+04;"#$+.G9AB 7"#$+1E@F 789K$052552632435303305 41!1E@F0321300!F#$2513+6;16

!15254H419:+04;"#$.355236(4132331

";6545789="#$."#$+254519:+040 41!65@7,2230505351!541!2789K 4312H43L5451305466(1351 E@F31"#$21F#$2

:130525410315IJF+,D@7,6521,.C6AB 79:+04;"#$.,D95AB 7"#$..7AB 79:+04;"#$+

.6AB 7"#$+5513521(5234566;2522(

789M@656(30541!1IJF+,D230323055;3 1!16@7,6513531365;16!15254H41 9:+04;"#$.41!5309:+04;"#$+#2125562130 E@F@7,6521IJF+,D5541!6519:+04;"#$.

"#$.9:+04;"#$+"#$+4312H43 55431305466(1351031

$0530(101!15231130(157$D30(1 5 7$95525322335219:+04;"#$.;"#$+7:525!

536+,,.NO5555536+,.7?5256543530525!1 4535222233521305631110P

QRSTUVWXYZ[\]^_^`abcd_becefghij\kfe`lmadcnojlpqrstuvwxyze`dc{^|}rstuvw~yz{`^^c|}nojlpqrstjvwyz€m^|dc]rstjvw‚yzƒb^_|m„bc{…†‡ˆ‰Š‹Œ‰

Žuip^„^‘’`^„„bc{_l^pe``^„’ec]bc{_`dc„’e`_^`ƒd`bdc_„“”^_^`abc^]YZ[\ƒdm^„dc]•[–pec—]^cp^bc_^`ƒd„˜ZY„™d`^bc]bpd_^]“^dcš„_dc]d`]]^ƒbd_becef ce`adb›^]]d_df`ea _l`^^bc]^’^c]^c_^‘’^`ba^c_„}^dpld_^d„_bc_`b’bpd_^}d`^„leœc“Yfce_ƒb„b€^}^``e`€d`„d`^„ad^`_ldc„a€e„“

o`ec_b^`„bce^pmd`žbe„pb^cp^„Ÿœœœ“f`ec_b^`„be`{ £ dj\juŸ ema^¡Ÿs`_b^¢¢¡[n

Ždc_b’m]bdc]oe_bd]b„ istdc]rstt`dc„’e`_s„„d„

(7)

012345627899888 8 9 8 !"#$% &'(7)*+",-.'$%

&)*+", #"$% &'(7)*+(, "#/$% &)*+(,

&0123456,+88!!87!+#78978 78!'(7)*+")*+"778'(7 )*+()*+(88 ('!9989 8!'(7 )*+"77'(7)*+(

99888788!878 98 8 878998 89 8!"$% &'(7)*+",:$% &)*+", '($% &'(7)*+(,($% &)*+(,&012345;,<79 88987897788 =#>?)98 78 @8 8 '(7)*+")*+"

'(7)*+()*+(+88!!87 87A7!+' ! 7888!'(7 )*+"778'(7)*+(*77988 88!898 )*+")*+(+88878

!887'(7@898@8!!87 89 8!'(7)*+"&"$%,'(7)*+(

&'($%,778898 )*+"&:$%, )*+"&($%,98

BC DEFDDC GH

*78998 8878978IJ01234562 889888 7>*+'(7)*+")*+()*+

)*+")*+(89898 8=#>?)98 78 7K898 889878 8 7 8 &L 89((,8M8

@878NO/!8! 98988I6PQ61798 7878"7NO/-&012345R, 779!8M8 @87 888878!8 8)98 78&012345S,T888 8888!A8!)98 788988 8888777&L 89((,+88 !)98 78&012345S,8877988 9 989 8 878&L89"..:U88789 ("UV889(",IJ012345627899NO/"&'(7 )*+"'(7)*+()*+",NO/#&)*+(, @8 78!"7&'(7)*+"'(7)*+()*+",(7

&)*+(,&L 89((,+87 98!!87!88 7'(8)98 78!A!98 887 78!'(7)*+()*+(&012345WSXYZ,9

[C\F]^_`abcdefgfhijklgjmkmnopnmhqrilkstuqvwxyoz{|}~mhlk€f‚xyoz{|ƒ~€hffk‚stuqvwxyou{|E~„…rflkexyou{|B~†jfgr‡jk€ˆ‰Š‹ŒŽŒ‘’“z”

vf……‡f•–hf‡‡jk€gqfvmhhf‡–mkejk€ghlk‡–mhgfh†lhjlkg‡—˜fgfhijkfeabcd†l…rf‡lke™cš vmk›efkvfjkgfh†l…‡œba‡lhfjkejvlgfe—’flkž‡glkelheef†jlgjmkmn kmhil…jŸfeelglnhmi gqhffjkef–fkefkgf•–fhjifkg‡‚flvqlg…fl‡gjkghj–…jvlgf‚lhf‡qm k—ankmg†j‡j„…f‚fhhmh„lh‡lhf‡il……fhgqlk‡¡i„m…‡—

thmkgjfh‡jk’m…fvr…lh¢jm‡vjfkvf‡£   —nhmkgjfh‡jmh€ § ’l¡uduz£¤m…rif¥£yhgjf¦“¦¥cs

‘lkgj–rejlketmgjlej‡ ”yolkexyoohlk‡–mhgy‡‡l¡‡

(8)

012345367897837044357024304876739 393323673!"399#123622632"47136 4820478717042733223$ %86&'"#%()*+3 78717042,-. 7$ 39/8601.23639* 4323"7539)34-5206,-.60430.()2343"0$4193 75932640$3973443789:;<869$0276387"903 4-52640$"0443208678=%()2781238"306&'">?@AB;C)3 9347867"3680"084717080638"79944037808,-.

178678= 0 97= 2187201.2 2 632"47136 2

"0$37753038867=923"7D"&'"#%()*7871704 E86039/F3639*)3042063230 267326334$78364-5206*&GH 3639*86*I 86GHE86039/604$8%()*.()23J432236 78K"39926347536640$ $023438940J7$91932H047$00 39/789:;<)34-506*ILGH604&'"#%()*017836 73343432383622>?@AB;MN7227$794035932*&

*I86GH6334$783660432$3.()278=K"3992 789:;<'434$043$02+7837"4$334260401.286

&'"#%()*6334$7836278=034"399328K"3992343920 78=006=433$38734-5593640$!"3992789:;<

.03534$4+3667663438"37260861333834-50601.2 6334$7836604$8&'"#%()278=3!*5GHF

>?@AB;MO863K"399#1236222P*GH 3639* 869$028078717708*GHE86039/'434$043 36QR7867"3684-5K#%()S4-5K#%()*47006P5 7"096137867"753067=23"7D"70601.20462

$8&'"#%()*8680&'"#%()3639*482408=

"084234-5!TU!#&'"#%()S4-5!TU!#&'"#%()*

470208910LV7867"78=27=87D"89$043$06343 23"7D"70601.20462&'"#%()*)72D8678=2 6434"0440104367863386389278=17"7"39923J432278=

397=2187290837"739636"0$41934-5!TU!#

%()S4-5!TU!#%()*4700610&I'4326732083 23"7D"70601.20462&'"#%()*86#%()799"947672

$17=7'0464345976708060432"399#123648204 2233236$780"7663475753223"7D"9934076 04$0832)286)&7"4321243206&'"#%()*86

#%()'47323$39*FW3538134=3839*5'04&'"#

%()*)243X3"367=34038"7848204787177088)&

>?@AB;YZN[N789783743570226732789:;<0+7837"

4$33423436086783973443604)286)&7&'"#

%()604"0$47208)2044-5604)286)&4343238 D42&'"#%()86%()23"7D"+7837"5932>?@AB;YZO\]\

[O\],2360830178364-5238"799440378&'"676

^_`abcdefghijklkmnopqlorprstusrmvwnqpuxyvz{|}t~€‚ƒrmqp„k…†|}t~€‡‚ƒ„mkkp…†uxyvz{|}ty€ˆ‚ƒ‰Šwk…qpj|}ty€‹‚ƒŒokl…wop„Ž‘’“”•–’—˜™~š

zkŠŠk›œmkop„lvkzrmmkœrpjop„lmqpœrmlkmŒqmoqplžklkmnopkjfghiŒqŠwkqpjŸh  zrp¡jkpzkoplkmŒqŠ¢gf£qmkopjozqlkj˜kqp¤lqpjqmjjkŒoqlorprs prmnqŠo¥kjjqlqsmrn lvmkkopjkœkpjkplk›œkmonkpl†kqzvqlŠkqloplmoœŠozqlk†qmkvr¦pfsprlŒoo‰Šk†kmmrm‰qmqmknqŠŠkmlvqp§n‰rŠ

xmrplokmop˜rŠkzwŠqm¨orzokpzk©¦¦¦smrplokmorm„ ­ ˜q§yiy~©ªrŠwnk«©}mlok¬™¬«hu

—qploœwjoqpjxrloqjo š}tqpj|}ttmqpœrml}q§

(9)

0123456407891240270606102367070123621318106 21236732072 !

"#$"%&'( #$

)6345691576412*6426+4624091244*1236 027104344267,4271013840-./3+012340+012/

40012340012/2426407037721!456789:;<86 6=9670>12465446162687070?706279448626 17406!!91260*406627572*12409127037721 214-./3+012340+012/!@3?706277018427107 3696544270236226+427572*642710379106740!

A1615644*96186723*64266=9670732 07291576?706279448626234236018946723 2316 1 1691070 >1246 1 702662 214 76027B427101917666212364074*912670-./3 107404B072*40967B72*!.72?706279448626102C 402-666*9157613840-./3+012/40 012/70236366966026*6426+4624091244*!

.704*2366247364991434044*141661 18910660704049976211236>12400121702662!

DEFEEGE( %EH( %( FI'FEFJKJ$F

2364424991270236107101237427676 846454746*2364231723120666542710!

E&FL#M"#$FM( H&F( #$'

N.1067564067062362*!@O961862366=9678602 162640404*,6236424!@O40N.126236840792!N.

124706236070!@O40N.644049915623687226 56710!

P&$D( $Q

2376643406*236R075672*1S60236@7T427104

@7606.104271040236@7T427104U60261U1896260670 V6643TUUV 240U6!

E"W$#X%JDQKJ$F'

)62340?@44S10622740Y640+A4Y6?6840015446 664?10236840792!

FEH%JZ[\]^_`abc]d]e]fg`bdhijbgk_lfmbckhinopkqrstuv`dirstupwbdhgbq_`a`^hghacx

"yz{y|}~ P€‚ƒ%EFZ("„…†‡Kyˆ‡‰Š‹KŒy%ƒŽ|‚‰}|{‘’ Mˆ}‚“

F‰“”yˆ’Š("„…Œ }‰‚{‘ˆ‘zˆ“ Ly“

srmhlqbdh pp •–—˜\™š› œžŸ ¡ ¢£¤¥¢ ¦`dgb_libhg`mpšpš›

p™§•—¨©›

nª—¨©› «]q¬ghc oabhch^`hg`mpššv›

v–xv§•x¨©› ¦`d`bhg`mv­­–›

v­x®§nx¨©› ¯`d`°bi`hg`mpššv›

±\² ®p ®pxv®§šx­p—˜\™š› ³`]cp \k]bhg`mpšv®›

®–x–§•x™—˜\™š› ¦b^ hg`mpšš–›

®™x•§ªx®—˜\™š› ¦±qhmmc ¦b^ hg`mpšš–›

®•xv§nx™—˜\™š› \ªqhmmc

­pxª§–x­—˜\™š› ¯´r•– ¯ldhg`mpšvn›

®–x•§vpx˜\™š› ²µ¦p­• ¦kldjhha`_k]d°hg`mpšvp›

v•p§p®x–—˜\™š› ³pqhmmc ©]ab^]g]hg`mpšš–›

¶·²pš• šxv­® vx•v§šxp®—˜\™š› ³`]cp \k]bhg`mpšv®›

šxvn—˜\™š› ³pqhmmc «i`hg`mpšvš›

šxv­`dišxpš—˜\™š› µdi]lhg`mpšš–›

šx®­§šxšª—˜\™š› ¯´r•– ¯ldhg`mpšvn›

šxv•n—˜\™š› ²urp­ ²¸¹b°hahg`mpšv–›

šxv®®—˜\™š› ³ºv®•ª

šxvpš—˜\™š› –™š™q

šxpš§šxš•—˜\™š› ¦¦»rš™™ ¯]gk`bc]d°hg`mpšv®›

šxvp§šxšp—˜\™š› ¦¦»rpv•

šxp™§šxšn—˜\™š› ¦¦»rvšš

vx• šx–—¨©› «]q¬ghc oabhch^`hg`mpššv›

vx–—¨©› ¼bgqkbhhg`mv­­­›

vx®§šx¨b› ³pqhmmc ©]ab^]g]hg`mpšš–›

un ®x­—¨©› «]q¬ghc oabhch^`hg`mpššv›

ªx•—¨©› ¼bgqkbhhg`mv­­­›

vv™§pxš—¨b› ³pqhmmc ©]ab^]g]hg`mpšš–›

"yz{y|}~ P€‚ƒ%EF€("„…†‡Kyˆ‡‰Š‹KŒy%ƒŽ|‚‰}|{‘’ Mˆ}‚“

F‰“”yˆ’Š("„…Œ }‰‚{‘ˆ‘zˆ“ Ly“

srmhlqbdh pvš ppv—˜\™š› œžŸ ¡ ¢£¤¥¢ ¦`dgb_libhg`mpšpš›

pn­§pp—¨©›

ppš—¨©› «]q¬ghc ·bdhi`hg`mv­­­›

±\² v­™ •v™§ª–xª—˜\™š› ³pqhmmc ©]ab^]g]hg`mpšš–›

¶·²pš• vx™ ½vš—˜\™š› ³pqhmmc «i`hg`mpšvš›

oa]dgbhacbd©]mhqlm`a±b]cqbhdqhc¾jjjxea]dgbhacbdx]a° À ©`¬pšpv¾¿]ml^h–¾tagbqmhª®ª–™n

¦`dgb_lib`dio]gb`ibc ²tu`distuua`dc_]agtcc`¬c

Referenzen

ÄHNLICHE DOKUMENTE

In addition, an intensive investigation into the association and dissociation of this complex was conducted on an Isothermal titration calorimetry(ITC) platform

To identify and characterize potential GLUT5 ligands, we developed a whole-cell system based on a yeast strain deficient in fructose uptake, in which GLUT5 transport activity

The EBY.VW4000 cells transformed with plasmids encoding GLUT5tr S72Y , GLUT5tr S76I or Hxt7 were cultivated in YEP media containing 2% (w/v) fructose and 200 µg/ml of G418

A: UDP-Glc and B: UDP- Xyl transport activities of Golgi vesicles isolated from yeast cells transformed with either the empty vector control (C) or vector constructs

Efalizumab inhibits the binding of αLβ2 to ICAM-1, -2, and -3 (Boehncke 2007a), and it is also able to inhibit the mixed lymphocyte response, T cell

Given that MOB proteins can function together with Ste20-like kinases in yeast, fly, and human cells [10], we further expanded our study by addressing all human

2   Structure–function analysis of VEGF receptor activation and the role of coreceptors in angiogenic signaling 17   3   Inhibition of receptor activation by Designed

Therefore, more pure and active deglycosylated hE-LecEGF can be prepared in the future by large-scale production and purification by the same procedure for further structural