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In vitro Inhibition of Cholinesterases by Carbamates Ð A Kinetic Study Alena Komersova´

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In vitro Inhibition of Cholinesterases by Carbamates Ð A Kinetic Study Alena Komersova´

a,

*, Marke´ta Masopustova´

a

, Karel Komers

a

, Sˇa´rka Sˇteˇpa´nkova´

b

,

and Alexandr C ˇ egan

b

a Department of Physical Chemistry, Faculty of Chemical Technology, University of Pardubice, Na´m. Legiı´ 565, 532 10 Pardubice, Czech Republic.

Fax: +42 04 66 03 70 68.

E-mail: alena.komersova@upce.cz

b Department of Biological and Biochemical Sciences, University of Pardubice, Sˇtrossova 239,

530 02 Pardubice, Czech Republic

* Author for correspondence and reprint requests Z. Naturforsch.62 c, 305Ð307 (2007); received October 20/December 4, 2006

Kinetics and mechanism ofin vitrohydrolyses of ace- tylcholine and acetylthiocholine by carbamates were studied in a batch reactor at 25∞C, pH 8, and ionic strength of 0.11m. Every hydrolysis was monitored by 3Ð4 independent methods. All studied hydrolyses can be described by the model of competitive inhibition with an irreversible step (k3). A table of obtained average values of rate constants and discussion of the resultes are given.

Key words:Acetylcholine, Hydrolysis, Kinetics, Cholin- esterases

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