• Keine Ergebnisse gefunden

Identification of neoepitopes recognized by tumor-infiltrating lymphocytes (TILs) from patients with glioma

N/A
N/A
Info
Herunterladen
Protected

Academic year: 2022

Aktie "Identification of neoepitopes recognized by tumor-infiltrating lymphocytes (TILs) from patients with glioma"

Copied!
1
0
0

Wird geladen.... (Jetzt Volltext ansehen)

Jetzt herunterladen ( 1 Seite )

Volltext

(1)

Identification of neoepitopes recognized by tumor-infiltrating lymphocytes (TILs) from patients with glioma

www.oncotarget.com Oncotarget, Supplementary Materials

SUPPLEMENTARY MATERIALS

Supplementary Table 1: List of predicted mutated epitopes and the corresponding wildtype sequence for each patient based on whole-exome sequencing data, as well as the cytokine responses of TILs. Cytokine production is provided in pg/10

5

TILs/7 days corrected by spontaneous cytokine production. See Supplementary_Table_1

Supplementary Table 2: IFN-γ production by peripheral blood T cells from patient GBM-C to mutant and wildtype peptides

ID Patient code Wildtype sequence IFN-γ Wildtype (PBMCs) IFN-γ Mutant

(PBMCs) Mutated sequence Gene

1 GBM-C QTHTEPTGDSKSIKA 503.84 QTHTEPTVDSKSIKA MKI67

2 GBM-C PKMLVNFLAKNKSIS 141.67 240.2 PKMLVNFVAKNKSIS OR5T1

3 GBM-C CGNVRVNAIYGLIVA 1174.31 CGNVRVNTIYGLIVA OR52N1

4 GBM-C GMGCPANRIHTYVYE 34.95 GMGCPANQIHTYVYE OOSP2

5 GBM-C FFATVECVLLAAMAY 79.99 291.84 FFATVECILLAAMAY OR5P2

6 GBM-C EQEQSSKRLSYQGQS 172.36 EQEQSSKCLSYQGQS C1orf116

7 GBM-C GTTRLLSVLFM 55.96 180.01 GTTRLLSALFM CD55

8 GBM-C AKTKASPAGKLEARA 111.65 AKTKASPVGKLEARA FICD

9 GBM-C TDRALQNKGISAFLV 79.39 TDRALQNQGISAFLV ACADS

10 GBM-C DTPSIEKRFAYSFLQ 737.33 DTPSIEKLFAYSFLQ RYR2

11 GBM-C DQCSCCSPTRTEPMQ 647.17 194.59 DQCSCCSLTRTEPMQ VWF

12 GBM-C ESVESRVLPGPRHRH 308.58 ESVESRVMPGPRHRH DDX11L11

13 GBM-C TTDSHIAEEKEDVKR 466.4 TTDSHIAKEKEDVKR CCDC168

14 GBM-C DDIACMIGYRPCPWM 491.89 DDIACMIRYRPCPWM SLC6A10P

15 GBM-C CNGTWSEDVGNHTKF 143.15 CNGTWSENVGNHTKF LEPR

16 GBM-C NRREAEERFNKQSAS 346.22 NRREAEEWFNKQSAS KRT24

17 GBM-C FIHCPTENEPDLAQC 909.36 77.89 FIHCPTEKTSQTWPS BIRC5

18 GBM-C IVLILTSAILLGSRV 80.35 IVLILTSTILLGSRV DHRS7C

19 GBM-C DRMPGKTEPETAGPN 75.42 DRMPGKTDPETAGPN RASAL3

20 GBM-C SNPHLLSHPSEPLEL 134.79 382.94 SNPHLLSLPSEPLEL LILRB3

21 GBM-C SNPHLLSHPSEPLEL 34.5 SNPHLLSYPSEPLEL LILRB3

22 GBM-C YSLWEHSTKNHLQLE 427.08 YSLWEHSMKNHLQLE APOB

23 GBM-C RRFVSQETGNLYIAK 147.22 RRFVSQEMGNLYIAK CNTN6

24 GBM-C LVTETSSVSTGHATP 72.13 LVTETSSASTGHATP MUC4

25 GBM-C DPCSLPLDEGSCTAY 429.75 DPCSLPLGEGSCTAY COL7A1

26 GBM-C QVLHLLGPKLEADLE 38.58 QVLHLLGHKLEADLE QARS

27 GBM-C QPGSTHTTAFPDSTT 329.66 QPGSTHTRAFPDSTT MUC12

28 GBM-C HHHHHHHRHPQPATY 160.85 HHHHHHHHHPQPATY HOXA1

29 GBM-C LAFLAESCATLSQEQ 495.61 LAFLAESYATLSQEQ TBRG4

30 GBM-C LENLQIIRGNMYYEN 114.62 LENLQIIKGNMYYEN EGFR

31 GBM-C ASVDNPHVCRLLGIC 459.85 ASVDNPHMCRLLGIC EGFR

32 GBM-C GARGFFQARHLEMDA 136.1 GARGFFQVRHLEMDA FAM83H

33 GBM-C EFYYCTEDVLRMGGD 161.97 EFYYCTEVVLRMGGD ENTPD4

34 GBM-C TKVESLHEQHSGLKQ 81.13 175.68 TKVESLHAQHSGLKQ PTPN3

35 GBM-C VWYSILKDTKITCEE 579.42 VWYSILKGTKITCEE ASTN2

36 GBM-C LHILTTLQEPHDKHL 510.13 774.76 LHILTTLEEPHDKHL TSC1

37 GBM-C TLVKRPAEPGGPQEP 560.09 370.28 TLVKRPAKPGGPQEP ABCA2

38 GBM-C KEYHFISTEEMTRNI 225.72 266.65 KEYHFISMEEMTRNI MPP1

39 GBM-C TLRVDRKRKVSGDSS 11.92 TLRVDRKHKVSGDSS BCOR

40 GBM-C PLSQESEVEEPLSQE 518.55 235.92 PLSQESEMEEPLSQE VCX3A

41 GBM-E IREQEEMLREQEAQR 183.97 1015.07 IREQEEMIREQEAQR GOLGA6L2

PBMCs from patient GBM-C were exposed to the mutated or wildtype-peptides for a period of seven days. Cell culture supernatants were then collected for IFN-γ measurement by sandwich ELISA. Shown is the actual cytokine values in pg/104 PBMCs, corrected by spontaneous cytokine production.

Referenzen

Jetzt herunterladen ( PDF - 1 Seite - 430.96 KB )

ÄHNLICHE DOKUMENTE

Interplay of stromal tumor‑infiltrating lymphocytes, normal colonic mucosa, cancer‑associated fibroblasts, clinicopathological data and the immunoregulatory molecules of patients diagnosed with colorectal cancer

Moreover, we confirmed a strong positive correlation of fibronectin with Ki-67 proliferation antigen and we noted that PD-L1 was also present in the tumor stroma, which may

Identification of neoepitopes recognized by tumor-infiltrating lymphocytes (TILs) from patients with glioma

After seven days of culture, interferon gamma (IFN-γ), tumor necrosis factor alpha (TNF-α) and/or IL-17A production was measured by ELISA in culture supernatants, and used as

Supplementary Table 1: Search terms for each construct of interest.

“gross motor” OR “motor performan*” OR “Motor proficien*” OR “motor abilit*” OR “object manipulation” OR “motor coordination” OR “actual competen*” OR

Supplementary Table 1: Characteristics of patients with BD compared with all patients in the RISE registry.

Total patients in RISE represents patients in RISE, aged 18 years and older, at least 2 visits with >= 30 days apart during the study period with

Table S1. Whole exome sequencing data of 3 patients with WEE2 mutation.

c.G585C Forward cgtaaaacacatctgttggcaggcagccctcc Reverse ggagggctgcctgccaacagatgtgttttacg c.115_116insT Forward ggaggcttcgagcctaaaccccagagaag.

This paper reports on the identification of the molecule RhP and its dissociation energy as well as on an upper limit for the dis- sociation energy of the molecule PtP

and to study the electronic charge distribution around the oxygen atom by evaluating the field gradient at the site of an oxygen nucleus from the molecular wave- functions..

of the UniversitY, as well as the

"Community Medicine" aufgebaut. Ein Eckpfeiler dieses Schwerpunktes ist die Integration der Problemstellungen der Lehre, Forschung und medizinischen Versorgung.

The predicted magnitudes as well as signs of the relative mobili­ ties are satisfactorily borne out by the experiments

Klemm on his sixtieth birthday The phenomenon of electrotransport in a binary liquid alloy with perfectly miscible components is representable in terms of